Composite

Part:BBa_K769012:Design

Designed by: Mayumi Nakamura   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)


Promoter(high)-RBS-lux operon-DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 505
    Illegal NheI site found at 4780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5242
    Illegal XhoI site found at 2592
    Illegal XhoI site found at 3100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 684
    Illegal BsaI.rc site found at 1366


Design Notes

Promoter(high)-RBS-lux operon-DT


Source

Photobacterium kishitanii strain NCMB 844 lux-rib operon

References